Monday, February 1, 2010

Lecture, chapter 13 - Cloning and recombninant DNA

Today we finished the chapter on cloning and recombinant DNA technology.

We covered two of the "classic" ways to analyze DNA: Southern blots and DNA sequencing.

We covered the steps to perform a Southern blot, including the basics of agarose gel electrophoresis, and how to use RNA/DNA probes to find genes of interest.

When discussing DNA sequencing we focused on automated DNA sequencing through the dye-terminator sequencing method, which uses dideoxynucleotides with a fluorescent label.

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