We developed lab guide 02 of this quarter.
We used F2 genetic corn (corn cobs with yellow, red and purple kernels) to test whether the phenotypic ratios (yellow or red/purple kernels) follow the ratios expected from Mendelian inheritance. Any statistically significant departure from the expected values is considered as evidence of epistasis.
The hypothesis (no difference between observed and expected values) was tested using the chi-square statistic (χ^2):
χ^2 = Σ [(O-E)^2 / E ],
where O is the observed value, and E the expected value. The degrees of freedom (d.f.) are defined as n-1, where n is the number of phenotypic categories. The level of significance was 0.05.
The result (p < 0.05) showed a great departure from the expected values, indicating a strong epistasis.
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