Lecture, chapter 13 - Cloning and recombinant DNA

A cartoon version of how to ligate DNA into
plasmids as part of the cloning process
click image to see a full size pic
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Today we started covering chapter 13, an introduction to cloning and recombinant DNA technology. We have temporarily skipped the second half of the chapter on mutation, but we will get back to it after finishing the current chapter.

We talked about the definition of cloning, and the basic steps for cloning DNA, including the use of plasmids, YACs, and BACs. We also briefly reviewed the basics of constructing a genomic library and how to probe it when searching for a specific fragment of DNA.

On Thursday: We will talk about in vitro cloning (PCR), southern blots, and automated DNA sequencing.

Tomorrow: Bioethics debates...!!!

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