Although the actual lab will be a take-home lab, we set the stage to do the work we need to do in the couple of mapping genes labs we will have.
We covered concepts like gene mapping, genetic linkage, and genetic distance within a chromosome, and how they are correlated. We also introduced the concept of CentiMorgan (cM), a.k.a. Map Unit (m.u.), and how it is useful to map genes, based on the results of experimental crosses.
We also used a little applet developed by Paul Lewis, from the University of Connecticut, called demonstration of crossing over. With this application we did simulations on recombination events affecting two loci separated by variable genetic distances. We then introduced the concepts of parental haplotypes/gametes and recombinant haplotypes/gametes, as part of the more practical explanation of genetic linkage.
We also did a quick demo of the software to be used in this lab: DrosophiLab. Students will download this Windows-based free software to simulate experimental crosses in Drosophila melanogaster, and calculate genetic distances between different genes. The labguide is being written and will be posted soon.
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